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GH may be viewed as the primary anabolic hormone during stress and fasting, whereas insulin is the major anabolic hormone in the preprandial timeframe, and it is primarily stimulated by protein in response to a meal (24,25). When the rate of weight loss decreases, the reduction in adiposity, with an associated increase in fat oxidation, may occur. This may result in impaired thermogenesis from carbohydrate, resulting in higher fat oxidation. Our previous study demonstrated that obese, postmenopausal women with a body mass index of 27 kg/m2 lost an average of 22 kg of fat mass in a period of 9 wk (1). Sugars and fiber are highly lipogenic and are associated with an increase in insulin secretion (1,26,27). Therefore, we hypothesized that the high levels of circulating insulin might be a factor limiting energy expenditure during weight loss and maintaining metabolic health. We hypothesized that adipose tissue lipids would be associated with decreased insulin secretion during weight loss and maintained metabolic health; thus, these may represent a factor limiting energy expenditure during weight loss. Methods Experimental Approach to the Problem Subjects Thirty-eight overweight women (n = 24), with body mass indexes in the normal range (28,29), between 40 and 85 +/- 1 kg were recruited (Table 1). Subjects had no family history of diabetes, were nonobese and had no history of cardiovascular disease. Subjects were informed of the study after approval from the institutional review board. We excluded women aged 35-71 y from inclusion in the study due to their low risk to develop coronary heart disease (30); additionally, we excluded women with severe obesity due to the high frequency of obesity-related comorbidities (1,31). Four of the subjects were Caucasian; all were obese at baseline and were randomly assigned to one of the four diets: diet-only, 3 wk of weight loss, 2 wk of weight loss and exercise and control (n = 19) or diet-plus-intervention (n = 20). All treatments consisted of 3 meals, 1 h per day, with 5 daily snacks. After a 12-wk washout period, the subjects resumed the same training program (32). Subjects in the intervention group were instructed to follow a diet restricted to high protein, low carbohydrate consumption for the duration of the study. The control group was instructed to follow the same standard American diet. Subjects in the other treatments were instructed to follow the standard diet (32). Training, rest, and food intake for the subjects and investigators were monitored by trained interviewers. Exclusion Criteria Subjects with any com Similar articles:
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